{"product_id":"methods-in-recombinant-dna-technology","title":"Methods in Recombinant DNA Technology","description":"Author : Archana Rani \u0026amp; Yogendra Singh\u003cbr\u003ePages : 138\u003cbr\u003eYear of Publication : 2026\u003cbr\u003eLanguage : English\u003cbr\u003eBinding : Paperback\u003cbr\u003eThis lab-focused manual converts your 10 practicals into step-by-step SOPs with checklists, calculations, controls, and troubleshooting—exactly what exams and vivas look for. Each exercise (growth media → buffers → miniprep → QC → digestion → gel → competent cells → E. coli transformation → screening → confirmation) includes:\r\n\r\n\r\n\t\r\n\tMaterials \u0026amp; prep tables (reagent recipes, stock→working dilutions, pH setpoints).\r\n\t\r\n\t\r\n\tCritical parameters (temperatures, times, rpm, enzyme units, percent agarose).\r\n\t\r\n\t\r\n\tControls (positive\/negative, vector-only, undigested DNA).\r\n\t\r\n\t\r\n\tResult criteria (acceptance ranges: A260\/280, band sizes, colony patterns).\r\n\t\r\n\t\r\n\tViva boxes (why steps work: alkaline lysis chemistry, star activity, blue-white logic).\r\n\t\r\n\t\r\n\tTroubleshooting maps (no colonies, smeared gels, low DNA yield, non-specific PCR).\r\n\t\r\n\r\n\r\nMini-numericals appear where needed: A260 quant, insert:vector molar ratio, digestion setup, spray-and-plate volumes, and transformation efficiency.\r\n","brand":"NIPA","offers":[{"title":"Default Title","offer_id":51505479090474,"sku":"NIPA-9367557892","price":32.99,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0693\/1218\/4618\/files\/NIPA1757679867.jpg?v=1778070868","url":"https:\/\/crazyshelf.com\/products\/methods-in-recombinant-dna-technology","provider":"Crazyshelf.com","version":"1.0","type":"link"}