1
/
of
1
Methods in Recombinant DNA Technology
Methods in Recombinant DNA Technology
Regular price
$32.99 USD
Regular price
$54.98 USD
Sale price
$32.99 USD
Unit price
/
per
Couldn't load pickup availability
Best Seller: #1 in Popular Products!
Fast Shipping
24/7 support
30 days return
Author : Archana Rani & Yogendra Singh
Pages : 138
Year of Publication : 2026
Language : English
Binding : Paperback
This lab-focused manual converts your 10 practicals into step-by-step SOPs with checklists, calculations, controls, and troubleshooting—exactly what exams and vivas look for. Each exercise (growth media → buffers → miniprep → QC → digestion → gel → competent cells → E. coli transformation → screening → confirmation) includes: Materials & prep tables (reagent recipes, stock→working dilutions, pH setpoints). Critical parameters (temperatures, times, rpm, enzyme units, percent agarose). Controls (positive/negative, vector-only, undigested DNA). Result criteria (acceptance ranges: A260/280, band sizes, colony patterns). Viva boxes (why steps work: alkaline lysis chemistry, star activity, blue-white logic). Troubleshooting maps (no colonies, smeared gels, low DNA yield, non-specific PCR). Mini-numericals appear where needed: A260 quant, insert:vector molar ratio, digestion setup, spray-and-plate volumes, and transformation efficiency.
Pages : 138
Year of Publication : 2026
Language : English
Binding : Paperback
This lab-focused manual converts your 10 practicals into step-by-step SOPs with checklists, calculations, controls, and troubleshooting—exactly what exams and vivas look for. Each exercise (growth media → buffers → miniprep → QC → digestion → gel → competent cells → E. coli transformation → screening → confirmation) includes: Materials & prep tables (reagent recipes, stock→working dilutions, pH setpoints). Critical parameters (temperatures, times, rpm, enzyme units, percent agarose). Controls (positive/negative, vector-only, undigested DNA). Result criteria (acceptance ranges: A260/280, band sizes, colony patterns). Viva boxes (why steps work: alkaline lysis chemistry, star activity, blue-white logic). Troubleshooting maps (no colonies, smeared gels, low DNA yield, non-specific PCR). Mini-numericals appear where needed: A260 quant, insert:vector molar ratio, digestion setup, spray-and-plate volumes, and transformation efficiency.
Share
